World Journal of Oncology, ISSN 1920-4531 print, 1920-454X online, Open Access |

Article copyright, the authors; Journal compilation copyright, World J Oncol and Elmer Press Inc |

Journal website http://www.wjon.org |

Original Article

Volume 10, Number 3, June 2019, pages 132-141

**Effects of p53 Status of Tumor Cells and Combined Treatment With Mild Hyperthermia, Wortmannin or Caffeine on Recovery From Radiation-Induced Damage**

Figures

Tables

Treatment | SAS/neo | SAS/mp53 |
---|---|---|

^{a}Numbers in parentheses are 95% confidence limits, determined using mean values, standard deviations and the numbers of observations on which the means and standard deviations were based. The difference between the values for SAS/neo and SAS/mp53 is statistically significant under each set of condition (P < 0.05). MHT: mild hyperthermia. | ||

Plating efficiency (%) | ||

Total tumor cells | ||

None | 48.4 (39.3 - 57.5)^{a} | 28.5 (22.4 - 34.6) |

MHT | 43.2 (39.3 - 47.1) | 20.9 (18.0 - 23.8) |

Caffeine | 28.3 (24.3 - 32.3) | 17.9 (14.9 - 20.9) |

Wortmannin | 28.6 (25.3 - 31.9) | 18.1 (15.6 - 20.6) |

Micronucleus frequency | ||

Total tumor cells | ||

None | 0.038 (0.032 - 0.044) | 0.072 (0.064 - 0.080) |

MHT | 0.050 (0.045 - 0.055) | 0.075 (0.068 - 0.082) |

Caffeine | 0.088 (0.079 - 0.097) | 0.137 (0.127 - 0.147) |

Wortmannin | 0.086 (0.078 - 0.094) | 0.136 (0.120 - 0.152) |

Quiescent tumor cells | ||

None | 0.056 (0.049 - 0.063) | 0.10 (0.09 - 0.11) |

MTH | 0.059 (0.053 - 0.065) | 0.10 (0.09 - 0.11) |

Caffeine | 0.114 (0.105 - 0.122) | 0.177 (0.164 - 0.190) |

Wortmannin | 0.116 (0.103 - 0.129) | 0.181 (0.161 - 0.201) |

SAS/neo | SAS/mp53 | |
---|---|---|

^{a}The dose of radiation required to obtain each endpoint with each treatment in relation to that required to obtain each endpoint immediately after HDR. ^{b}Numbers in parentheses are 95% confidence limits, determined using mean values, standard deviations and the numbers of observations on which the means and standard deviations were based. Except for the conditions using treatment with wortmannin, the difference between the values for SAS/neo and SAS/mp53 is statistically significant under each set of condition (P < 0.05). HDR: high dose-rate irradiation; MHT: mild hyperthermia; WM: wortmannin; CAF: caffeine; LDR: low dose-rate irradiation. | ||

Surviving fraction = 0.04 | ||

Total tumor cells | ||

Right after HDR | 1.0 | 1.0 |

9 h after HDR | 1.3 (1.2 - 1.4)^{b} | 1.05 (1.0 - 1.1) |

MHT right after HDR, 9 h later | 1.2 (1.1 - 1.3) | 1.04 (1.01 - 1.07) |

WM right after HDR, 9 h later | 1.05 (1.0 - 1.1) | 1.02 (1.0 - 1.05) |

CAF right after HDR, 9 h later | 1.25 (1.2 - 1.3) | 1.05 (1.0 - 1.1) |

After LDR | 1.4 (1.3 - 1.5) | 1.1 (1.0 - 1.1) |

LDR with MHT | 1.25 (1.2 - 1.3) | 1.06 (1.04 - 1.08) |

LDR with WM | 1.05 (1.0 - 1.1) | 1.05 (1.0 - 1.1) |

LDR with CAF | 1.35 (1.3 - 1.4) | 1.07 (1.05 - 1.1) |

Net micronucleus frequency = 0.1 | ||

Total tumor cells | ||

Right after HDR | 1.0 | 1.0 |

9 h after HDR | 1.25 (1.3 - 1.4) | 1.05 (1.0 - 1.1) |

MHT right after HDR, 9 h later | 1.2 (1.1 - 1.3) | 1.04 (1.0 - 1.08) |

WM right after HDR, 9 h later | 1.1 (1.0 - 1.2) | 1.03 (1.0 - 1.05) |

CAF right after HDR, 9 h later | 1.25 (1.2 - 1.3) | 1.05 (1.0 - 1.1) |

After LDR | 1.3 (1.2 - 1.4) | 1.1 (1.0 - 1.2) |

LDR with MHT | 1.25 (1.2 - 1.3) | 1.06 (1.04 - 1.08) |

LDR with WM | 1.1 (1.0 - 1.2) | 1.03 (1.0 - 1.05) |

LDR with CAF | 1.3 (1.2 - 1.4) | 1.07 (1.05 - 1.1) |

Quiescent tumor cells | ||

Right after HDR | 1.0 | 1.0 |

9 h after HDR | 1.5 (1.4 - 1.6) | 1.07 (1.05 - 1.1) |

MHT right after HDR, 9 h later | 1.25 (1.2 - 1.3) | 1.05 (1.0 - 1.1) |

WM right after HDR, 9 h later | 1.1 (1.0 - 1.2) | 1.02 (1.0 - 1.05) |

CAF right after HDR, 9 h later | 1.35 (1.3 - 1.4) | 1.07 (1.04 - 1.1) |

After LDR | 1.65 (1.6 - 1.7) | 1.15 (1.1 - 1.2) |

LDR with MHT | 1.3 (1.2 - 1.4) | 1.07 (1.04 - 1.1) |

LDR with WM | 1.1 (1.0 - 1.2) | 1.02 (1.0 - 1.05) |

LDR with CAF | 1.45 (1.4 - 1.5) | 1.1 (1.0 - 1.1) |

SAS/neo | SAS/mp53 | |
---|---|---|

^{a}The dose of radiation required to obtain a net micronucleus frequency of 0.1 in quiescent tumor cells in relation to that required to obtain a net micronucleus frequency of 0.1 in the total tumor cell population. ^{b}Numbers in parentheses are 95% confidence limits, determined using mean values, standard deviations and the numbers of observations on which the means and standard deviations were based. The difference between the values for SAS/neo and SAS/mp53 is statistically significant under each set of condition (P < 0.05). HDR: high dose-rate irradiation; MHT: mild hyperthermia; WM: wortmannin; CAF: caffeine; LDR: low dose-rate irradiation. | ||

Net micronucleus frequency = 0.1 | ||

Right after HDR | 2.2 (2.1 - 2.3)^{b} | 1.9 (1.8 - 2.0) |

9 h after HDR | 2.35 (2.25 - 2.45) | 1.95 (1.85 - 2.05) |

MHT right after HDR, 9 h later | 2.2 (2.1 - 2.3) | 1.9 (1.8 - 2.0) |

WM right after HDR, 9 h later | 2.1 (2.0 - 2.2) | 1.85 (1.75 - 1.95) |

CAF right after HDR, 9 h later | 2.3 (2.2 - 2.4) | 1.9 (1.8 - 2.0) |

After LDR | 2.35 (2.25 - 2.45) | 1.95 (1.85 - 2.05) |

LDR with MHT | 2.2 (2.1 - 2.3) | 1.9 (1.8 - 2.0) |

LDR with WM | 2.1 (2.0 - 2.2) | 1.85 (1.75 - 1.95) |

LDR with CAF | 2.3 (2.2 - 2.4) | 1.9 (1.8 - 2.0) |