World Journal of Oncology, ISSN 1920-4531 print, 1920-454X online, Open Access
Article copyright, the authors; Journal compilation copyright, World J Oncol and Elmer Press Inc
Journal website http://www.wjon.org

Original Article

Volume 11, Number 1, February 2020, pages 1-8

Inclusion of an E7 DNA Amplification Test Improves the Robustness of Human Papillomavirus-Associated Oropharyngeal Squamous Cell Carcinoma Diagnosis

Figures

Figure 1.
Figure 1. Tonsil specimen immunostained for p16. FFPE section from a tonsil scored negative for p16, with few focally positive stained cells. Magnification bar = 50 µm. FFPE: formalin fixed paraffin embedded.
Figure 2.
Figure 2. Base of the tongue specimen immunostained for p16. FFPE section from the base of the tongue scored positive for p16, with greater than 70% of nuclei and cytoplasm of tumor cells stained positive. Magnification bar = 50 µm. FFPE: formalin fixed paraffin embedded.
Figure 3.
Figure 3. Gel electrophoresis of HPV16 E7 and HPV18 E7 PCR amplicons. Indicated samples were electrophoresed with a 100 base pair amplicon sizing ladder on a 2% agarose gel containing ethidium bromide. HPV: human papillomavirus; PCR: polymerase chain reaction.

Tables

Table 1. Patient and Tumor Specimen Characteristics
 
aSelf identified.
Age range (years)52 - 86
SexMale
Racea (number of patients)
  White10
  Black8
  Unknown3
Anatomic site (number of specimens)
  Oropharynx17
  Lymph node2
  Floor of mouth1
  Hypopharynx1
HistologySquamous cell carcinoma

 

Table 2. Results of p16 Immunostaining, High-Risk (HPV16/18) DNA ISH, and DNA PCR for HPV16 E7 for Each Patient Specimen Tested
 
Specimenp16 IHCHPV16/18 ISHHPV16 E7 PCRResultsa
aNumber of specimens tested with similar results. bTest not done. DNA: deoxyribonucleic acid; IHC: immunohistochemistry; HPV: human papillomavirus; ISH: in situ hybridization; PCR: polymerase chain reaction.
Base of tongue+++3
Base of tongue+-+4
Base of tongue+--2
Tonsil+-+1
Tonsil---1
Tonsil+++5
Tonsil+-+1
Hypopharynx---1
Lymph node+++1
Lymph node+NDb+1
Floor of mouth-NDb+1