World Journal of Oncology, ISSN 1920-4531 print, 1920-454X online, Open Access
Article copyright, the authors; Journal compilation copyright, World J Oncol and Elmer Press Inc
Journal website http://www.wjon.org

Original Article

Volume 1, Number 1, February 2010, pages 7-13

Targeting RhoC by Way of Ribozyme Trangene in Human Breast Cancer Cells and its Impact on Cancer Invasion

Figures

Figure 1.
Figure 1. Secondary structure of human RHO-C mRNA, folded using Zuker’s programme [24]. Arrows: target sites for the ribozyme transgenes.
Figure 2.
Figure 2. RT-PCR showing MDA-MB-231 wild type cells expressed RhoC transcript. Left: Retroviral transgenes pRevTre-RhoC successfully knocked down expression of the RhoC transcript from the MDA-MB-231 breast cancer cells. Right: anti-Rho-C transgenes constructed using the pEZO EcoSpe vector.
Figure 3.
Figure 3. Graphical representation of the effect of a panel of ribozymes to RhoC on invasion of MDA-MB-231 breast cancer cells compared with MDA-MB-231WT and MDA-MB-231pRevTRE cells. Top left: MDA-MB-231ΔRHOC1; Middle: MDA-MB-231ΔRHOC2 and bottom: MDA-MB-231ΔRHOC3.