Figures
![Figure 1.](/tables/wjon2010.03.195w-g001.jpg)
Figure 1. The effect of sesamin on cell cytotoxicity on human leukemic cell lines. Cell viability of human leukemic HL-60, U937 and Molt-4 cells were compared to PBMCs treated with sesamin at various concentrations by using MTT assay.
![Figure 2.](/tables/wjon2010.03.195w-g002.jpg)
Figure 2. Cell morphology of U937 cells after treatment with sesamin. The cells were treated with sesamin at indicated concentrations for 24 h and stained with propidium iodide and examined under fluorescence microscope. Condensed nuclei and apoptotic bodies were marked with arrows. Magnificaiton x 400.
![Figure 3.](/tables/wjon2010.03.195w-g003.jpg)
Figure 3. Dot plots and histograms of HL-60 cells treated with sesamin. The cells were stained with 40 nM of 3,3'-dihexyloxacarbocyanine iodide and detected by flow cytometry. M1 area represents percentage of cells with decreased MTP. Circle area is cells with smaller size which are apoptotic cells.
![Figure 4.](/tables/wjon2010.03.195w-g004.jpg)
Figure 4. The reduction of mitochondrial transmembrane potential of U937 cells treated with sesamin for 4 h. Percent cells with reduced MTP were increased in a dose response manner. The data were means ± S.E.M. of three independent experiments performed in duplicate. p < 0.05, (*) significant difference from control.
![Figure 5.](/tables/wjon2010.03.195w-g005.jpg)
Figure 5. The reduction of MTP of HL-60 cells when treated with sesamin for 1 h. The histograms of cells when treated with H2O2, sesamin at 40 ug/ml and combined treatment (upper panel) and percentages of cells with decreased MTP in each condition (lower panel) are shown.
![Figure 6.](/tables/wjon2010.03.195w-g006.jpg)
Figure 6. The effect of sesamin treatment on ROS production in human leukemic cells. Dot plot and histogram of HL-60 cells treated with sesamin at indicated concentrations for 4 h. The cells that stained with DCFH-DA were cells producing ROS and existed under area M1. It was in a dose response manner of increasing in production of free radicals.
![Figure 7.](/tables/wjon2010.03.195w-g007.jpg)
Figure 7. The effect of sesamin on ROS production in Molt-4 cells and HL-60. The Molt-4 (A) and HL-60 (B) cells were treated with sesamin at indicated concentrations for 4 h and in combined treatment with H2O2. Positive control was the treatment with 15 mM H2O2 alone. The data were performed in three independent experiments and represented as mean ± S.E.M. p < 0.05 (*) is considered as significant difference compared to control.
![Figure 8.](/tables/wjon2010.03.195w-g008.jpg)
Figure 8. The effect of sesamin on caspase-3 activity of HL-60 cells. An increase of caspase-3 activity of HL-60 cells treated with sesamin at indicated concentrations are determined by DEVD-AMC substrate. Naringenin at 250 ug/ml was used as positive control for caspase-3 activity induction in HL-60 cells.
![Figure 9.](/tables/wjon2010.03.195w-g009.jpg)
Figure 9. GADD153 expression of sesamin-treated HL-60 cells. The cells were treated with sesamin 40 ug/ml at indicated times. The upper panel shows the bands from immunoblot of GADD153 compared to actin as a constitutive protein. The lower panel represents the data as mean ± S.E.M. p < 0.05 (*) considered significant difference compared to control.